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human hepatocellular carcinoma cells hcc36  (ATCC)


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    ATCC human hepatocellular carcinoma cells hcc36
    Specific retention of TAT-ΔNS3/4A-FITC in <t>NS3/4A-HCC36-</t> and HCV-infected cells. HCC36 cells, NS3/4A-HCC36 cells, or JFH-1-infected HCC36 cells were incubated with 10 μM TAT-ΔNS3/4A-FITC in the absence or presence of 2 μM telaprevir (NS3/4A protease inhibitor) at 37°C for 1 h. The cells were washed with DMEM containing 10% serum three times per hour. After culturing for 1 or 8 h, the phase contrast and fluorescence of viable cells were observed under a fluorescence microscope. Scale bar: 100 μm.
    Human Hepatocellular Carcinoma Cells Hcc36, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3826 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hepatocellular+carcinoma+cells+hcc36/Hep+G2%3B+Hepatocellular%3B+Carcinoma%3B+Human/pmc09672079-34-0-5
    Average 99 stars, based on 3826 article reviews
    human hepatocellular carcinoma cells hcc36 - by Bioz Stars, 2026-10
    99/100 stars

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    1) Product Images from "Micro-PET imaging of hepatitis C virus NS3/4A protease activity using a protease-activatable retention probe"

    Article Title: Micro-PET imaging of hepatitis C virus NS3/4A protease activity using a protease-activatable retention probe

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2022.896588

    Specific retention of TAT-ΔNS3/4A-FITC in NS3/4A-HCC36- and HCV-infected cells. HCC36 cells, NS3/4A-HCC36 cells, or JFH-1-infected HCC36 cells were incubated with 10 μM TAT-ΔNS3/4A-FITC in the absence or presence of 2 μM telaprevir (NS3/4A protease inhibitor) at 37°C for 1 h. The cells were washed with DMEM containing 10% serum three times per hour. After culturing for 1 or 8 h, the phase contrast and fluorescence of viable cells were observed under a fluorescence microscope. Scale bar: 100 μm.
    Figure Legend Snippet: Specific retention of TAT-ΔNS3/4A-FITC in NS3/4A-HCC36- and HCV-infected cells. HCC36 cells, NS3/4A-HCC36 cells, or JFH-1-infected HCC36 cells were incubated with 10 μM TAT-ΔNS3/4A-FITC in the absence or presence of 2 μM telaprevir (NS3/4A protease inhibitor) at 37°C for 1 h. The cells were washed with DMEM containing 10% serum three times per hour. After culturing for 1 or 8 h, the phase contrast and fluorescence of viable cells were observed under a fluorescence microscope. Scale bar: 100 μm.

    Techniques Used: Infection, Incubation, Protease Inhibitor, Fluorescence, Microscopy

    Specific retention of TAT-ΔNS3/4A-FITC in NS3/4A-expressing tumors in vivo . Mice bearing established NS3/4A-HCC36 and HCC36 tumors were injected with 500 μM TAT-NS3/4A-FITC and sacrificed after 4 h. Sections of NS3/4A-HCC36 (upper panels) and HCC36 (lower panels) tumors were stained with 520 HCV protease Assay Kit (AnaSpec) to detect NS3/4A activity in tumor sections. FITC-derived fluorescence (green) and NS3/4A activity (red) were observed under a fluorescence microscope. Scale bar: 1 mm.
    Figure Legend Snippet: Specific retention of TAT-ΔNS3/4A-FITC in NS3/4A-expressing tumors in vivo . Mice bearing established NS3/4A-HCC36 and HCC36 tumors were injected with 500 μM TAT-NS3/4A-FITC and sacrificed after 4 h. Sections of NS3/4A-HCC36 (upper panels) and HCC36 (lower panels) tumors were stained with 520 HCV protease Assay Kit (AnaSpec) to detect NS3/4A activity in tumor sections. FITC-derived fluorescence (green) and NS3/4A activity (red) were observed under a fluorescence microscope. Scale bar: 1 mm.

    Techniques Used: Expressing, In Vivo, Injection, Staining, Protease Assay, Activity Assay, Derivative Assay, Fluorescence, Microscopy

    Specificity and half-life of TAT-ΔNS3/4A- 124 I-FITC. (A) NS3/4A-HCC36 and HCC36 cells were incubated with 37 kBq of TAT-ΔNS3/4A- 124 I-FITC in the presence or absence of 2 μM Telaprevir at 37°C for 1 h. The cells were washed with DMEM containing 10% serum three times per hour. The cells were collected by treatment with trypsin at the indicated times. The radioactivity of the cells was then measured with a gamma-counter. The CPM was normalized by protein concentration. (B) Kinetics of the TAT-ΔNS3/4A- 124 I-FITC in vivo , BALB/c mice were intravenously injected with TAT-ΔNS3/4A- 124 I-FITC, and the radioactivity in serum samples collected at the indicated times was measured using a gamma-counter. t 1/2 = 2.55 min. Error bar: standard error of triplicate determinations.
    Figure Legend Snippet: Specificity and half-life of TAT-ΔNS3/4A- 124 I-FITC. (A) NS3/4A-HCC36 and HCC36 cells were incubated with 37 kBq of TAT-ΔNS3/4A- 124 I-FITC in the presence or absence of 2 μM Telaprevir at 37°C for 1 h. The cells were washed with DMEM containing 10% serum three times per hour. The cells were collected by treatment with trypsin at the indicated times. The radioactivity of the cells was then measured with a gamma-counter. The CPM was normalized by protein concentration. (B) Kinetics of the TAT-ΔNS3/4A- 124 I-FITC in vivo , BALB/c mice were intravenously injected with TAT-ΔNS3/4A- 124 I-FITC, and the radioactivity in serum samples collected at the indicated times was measured using a gamma-counter. t 1/2 = 2.55 min. Error bar: standard error of triplicate determinations.

    Techniques Used: Incubation, Radioactivity, Protein Concentration, In Vivo, Injection

    Micro-PET imaging of NS3/4A activity in vivo . (A) Mice bearing established NS3/4A-HCC36 (right hind leg) and HCC36 (left hind leg) tumors were injected with 3,700 kBq of TAT-ΔNS3/4A- 124 I-FITC. Coronal and transverse images were acquired at 2, 4, and 6 h after injection of the probe. (B) Mice bearing established NS3/4A-HCC36 (right hind leg) and HCC36 (left hind leg) tumors were intraperitoneally injected with telaprevir (20 mg/kg/day for 3 days) before intravenous injection of TAT-ΔNS3/4A- 124 I-FITC (3,700 kBq). Coronal images of tumor sections were acquired at 2, 4, and 6 h after injection of the probe.
    Figure Legend Snippet: Micro-PET imaging of NS3/4A activity in vivo . (A) Mice bearing established NS3/4A-HCC36 (right hind leg) and HCC36 (left hind leg) tumors were injected with 3,700 kBq of TAT-ΔNS3/4A- 124 I-FITC. Coronal and transverse images were acquired at 2, 4, and 6 h after injection of the probe. (B) Mice bearing established NS3/4A-HCC36 (right hind leg) and HCC36 (left hind leg) tumors were intraperitoneally injected with telaprevir (20 mg/kg/day for 3 days) before intravenous injection of TAT-ΔNS3/4A- 124 I-FITC (3,700 kBq). Coronal images of tumor sections were acquired at 2, 4, and 6 h after injection of the probe.

    Techniques Used: Micro-PET, Imaging, Activity Assay, In Vivo, Injection

    Biodistribution of TAT-ΔNS3/4A- 124 I-FITC in xenograft mice. Mice bearing established NS3/4A-HCC36 and HCC36 tumors were injected with 3,700 kBq of TAT-ΔNS3/4A- 124 I-FITC. Selected organs and tumors were removed from the mice after 2 (white column), 4 (black column), and 6 (gray column) h. The radioactivity of individual organs was measured using a gamma-counter and normalized for sample weights. The biodistribution of TAT-ΔNS3/4A- 124 I-FITC in selected organs was expressed as percentage injected dose/g tissue. Data represent mean ± SEM.
    Figure Legend Snippet: Biodistribution of TAT-ΔNS3/4A- 124 I-FITC in xenograft mice. Mice bearing established NS3/4A-HCC36 and HCC36 tumors were injected with 3,700 kBq of TAT-ΔNS3/4A- 124 I-FITC. Selected organs and tumors were removed from the mice after 2 (white column), 4 (black column), and 6 (gray column) h. The radioactivity of individual organs was measured using a gamma-counter and normalized for sample weights. The biodistribution of TAT-ΔNS3/4A- 124 I-FITC in selected organs was expressed as percentage injected dose/g tissue. Data represent mean ± SEM.

    Techniques Used: Injection, Radioactivity

    Micro-PET Imaging of NS3/4A activity in orthotopic liver implantation model. The NS3/4A-HCC36 tumors or HCC36 tumors from the ectopic tumors were harvested and transplanted into the left liver lobe of SCID mice ( n = 3). After 2 weeks, the 3,700 kBq of TAT-ΔNS3/4A- 124 I-FITC was intravenously injected. (A) PET imaging was performed at 4 h after injection of the probe. (B) The accumulation of radioactivity in NS3/4A-HCC36 tumors and HCC36 tumors was measured using a gamma-counter at 2 (white column), 4 (black column), and 6 (gray column) h after probe injection. Data represent mean ± SEM. Student’s t -test analysis of data. Statistical analysis was compared NS3/4A-HCC36 with HCC36. P < 0.05 was considered statistically significant.
    Figure Legend Snippet: Micro-PET Imaging of NS3/4A activity in orthotopic liver implantation model. The NS3/4A-HCC36 tumors or HCC36 tumors from the ectopic tumors were harvested and transplanted into the left liver lobe of SCID mice ( n = 3). After 2 weeks, the 3,700 kBq of TAT-ΔNS3/4A- 124 I-FITC was intravenously injected. (A) PET imaging was performed at 4 h after injection of the probe. (B) The accumulation of radioactivity in NS3/4A-HCC36 tumors and HCC36 tumors was measured using a gamma-counter at 2 (white column), 4 (black column), and 6 (gray column) h after probe injection. Data represent mean ± SEM. Student’s t -test analysis of data. Statistical analysis was compared NS3/4A-HCC36 with HCC36. P < 0.05 was considered statistically significant.

    Techniques Used: Micro-PET, Imaging, Activity Assay, Injection, Radioactivity

    Related Articles

    Cell Culture:

    Article Title: Micro-PET imaging of hepatitis C virus NS3/4A protease activity using a protease-activatable retention probe
    Article Snippet: .. Human hepatocellular carcinoma cells HCC36 (American Type Culture Collection, Manassas, VA, United States) were cultured in Dulbecco’s Minimal Essential Medium (Sigma-Aldrich, Burlington, MA, United States) supplemented with 10% heat-inactivated bovine calf serum, 100 units/ml penicillin, and 100 μg/ml streptomycin (Sigma-Aldrich, Burlington, MA, United States) at 37°C in a humidified atmosphere of 5% CO2. .. NS3/4A-HCC36 cell lines by transfection of recombinant lentivirus consist package of plasmid: pCMVΔR8.91 (RNAi core, Academia Sinica, Taipei, Taiwan) and pMD.G (RNAi core, Academia Sinica, Taipei, Taiwan) and target plasmid: pLKO_AS3 NS3/4A, respectively; after selection by 1, 3, and 5 μg/ml puromycin dihydrochloride (P8833, Sigma-Aldrich, Burlington, MA, United States), the selection medium was replaced every 2 days for 1 week.



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    human hepatocellular carcinoma cells hcc36  (ATCC)
    99
    ATCC human hepatocellular carcinoma cells hcc36
    Specific retention of TAT-ΔNS3/4A-FITC in <t>NS3/4A-HCC36-</t> and HCV-infected cells. HCC36 cells, NS3/4A-HCC36 cells, or JFH-1-infected HCC36 cells were incubated with 10 μM TAT-ΔNS3/4A-FITC in the absence or presence of 2 μM telaprevir (NS3/4A protease inhibitor) at 37°C for 1 h. The cells were washed with DMEM containing 10% serum three times per hour. After culturing for 1 or 8 h, the phase contrast and fluorescence of viable cells were observed under a fluorescence microscope. Scale bar: 100 μm.
    Human Hepatocellular Carcinoma Cells Hcc36, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hepatocellular+carcinoma+cells+hcc36/Hep+G2%3B+Hepatocellular%3B+Carcinoma%3B+Human/pmc09672079-34-0-5
    Average 99 stars, based on 1 article reviews
    human hepatocellular carcinoma cells hcc36 - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier

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    Specific retention of TAT-ΔNS3/4A-FITC in NS3/4A-HCC36- and HCV-infected cells. HCC36 cells, NS3/4A-HCC36 cells, or JFH-1-infected HCC36 cells were incubated with 10 μM TAT-ΔNS3/4A-FITC in the absence or presence of 2 μM telaprevir (NS3/4A protease inhibitor) at 37°C for 1 h. The cells were washed with DMEM containing 10% serum three times per hour. After culturing for 1 or 8 h, the phase contrast and fluorescence of viable cells were observed under a fluorescence microscope. Scale bar: 100 μm.

    Journal: Frontiers in Microbiology

    Article Title: Micro-PET imaging of hepatitis C virus NS3/4A protease activity using a protease-activatable retention probe

    doi: 10.3389/fmicb.2022.896588

    Figure Lengend Snippet: Specific retention of TAT-ΔNS3/4A-FITC in NS3/4A-HCC36- and HCV-infected cells. HCC36 cells, NS3/4A-HCC36 cells, or JFH-1-infected HCC36 cells were incubated with 10 μM TAT-ΔNS3/4A-FITC in the absence or presence of 2 μM telaprevir (NS3/4A protease inhibitor) at 37°C for 1 h. The cells were washed with DMEM containing 10% serum three times per hour. After culturing for 1 or 8 h, the phase contrast and fluorescence of viable cells were observed under a fluorescence microscope. Scale bar: 100 μm.

    Article Snippet: Human hepatocellular carcinoma cells HCC36 (American Type Culture Collection, Manassas, VA, United States) were cultured in Dulbecco’s Minimal Essential Medium (Sigma-Aldrich, Burlington, MA, United States) supplemented with 10% heat-inactivated bovine calf serum, 100 units/ml penicillin, and 100 μg/ml streptomycin (Sigma-Aldrich, Burlington, MA, United States) at 37°C in a humidified atmosphere of 5% CO2.

    Techniques: Infection, Incubation, Protease Inhibitor, Fluorescence, Microscopy

    Specific retention of TAT-ΔNS3/4A-FITC in NS3/4A-expressing tumors in vivo . Mice bearing established NS3/4A-HCC36 and HCC36 tumors were injected with 500 μM TAT-NS3/4A-FITC and sacrificed after 4 h. Sections of NS3/4A-HCC36 (upper panels) and HCC36 (lower panels) tumors were stained with 520 HCV protease Assay Kit (AnaSpec) to detect NS3/4A activity in tumor sections. FITC-derived fluorescence (green) and NS3/4A activity (red) were observed under a fluorescence microscope. Scale bar: 1 mm.

    Journal: Frontiers in Microbiology

    Article Title: Micro-PET imaging of hepatitis C virus NS3/4A protease activity using a protease-activatable retention probe

    doi: 10.3389/fmicb.2022.896588

    Figure Lengend Snippet: Specific retention of TAT-ΔNS3/4A-FITC in NS3/4A-expressing tumors in vivo . Mice bearing established NS3/4A-HCC36 and HCC36 tumors were injected with 500 μM TAT-NS3/4A-FITC and sacrificed after 4 h. Sections of NS3/4A-HCC36 (upper panels) and HCC36 (lower panels) tumors were stained with 520 HCV protease Assay Kit (AnaSpec) to detect NS3/4A activity in tumor sections. FITC-derived fluorescence (green) and NS3/4A activity (red) were observed under a fluorescence microscope. Scale bar: 1 mm.

    Article Snippet: Human hepatocellular carcinoma cells HCC36 (American Type Culture Collection, Manassas, VA, United States) were cultured in Dulbecco’s Minimal Essential Medium (Sigma-Aldrich, Burlington, MA, United States) supplemented with 10% heat-inactivated bovine calf serum, 100 units/ml penicillin, and 100 μg/ml streptomycin (Sigma-Aldrich, Burlington, MA, United States) at 37°C in a humidified atmosphere of 5% CO2.

    Techniques: Expressing, In Vivo, Injection, Staining, Protease Assay, Activity Assay, Derivative Assay, Fluorescence, Microscopy

    Specificity and half-life of TAT-ΔNS3/4A- 124 I-FITC. (A) NS3/4A-HCC36 and HCC36 cells were incubated with 37 kBq of TAT-ΔNS3/4A- 124 I-FITC in the presence or absence of 2 μM Telaprevir at 37°C for 1 h. The cells were washed with DMEM containing 10% serum three times per hour. The cells were collected by treatment with trypsin at the indicated times. The radioactivity of the cells was then measured with a gamma-counter. The CPM was normalized by protein concentration. (B) Kinetics of the TAT-ΔNS3/4A- 124 I-FITC in vivo , BALB/c mice were intravenously injected with TAT-ΔNS3/4A- 124 I-FITC, and the radioactivity in serum samples collected at the indicated times was measured using a gamma-counter. t 1/2 = 2.55 min. Error bar: standard error of triplicate determinations.

    Journal: Frontiers in Microbiology

    Article Title: Micro-PET imaging of hepatitis C virus NS3/4A protease activity using a protease-activatable retention probe

    doi: 10.3389/fmicb.2022.896588

    Figure Lengend Snippet: Specificity and half-life of TAT-ΔNS3/4A- 124 I-FITC. (A) NS3/4A-HCC36 and HCC36 cells were incubated with 37 kBq of TAT-ΔNS3/4A- 124 I-FITC in the presence or absence of 2 μM Telaprevir at 37°C for 1 h. The cells were washed with DMEM containing 10% serum three times per hour. The cells were collected by treatment with trypsin at the indicated times. The radioactivity of the cells was then measured with a gamma-counter. The CPM was normalized by protein concentration. (B) Kinetics of the TAT-ΔNS3/4A- 124 I-FITC in vivo , BALB/c mice were intravenously injected with TAT-ΔNS3/4A- 124 I-FITC, and the radioactivity in serum samples collected at the indicated times was measured using a gamma-counter. t 1/2 = 2.55 min. Error bar: standard error of triplicate determinations.

    Article Snippet: Human hepatocellular carcinoma cells HCC36 (American Type Culture Collection, Manassas, VA, United States) were cultured in Dulbecco’s Minimal Essential Medium (Sigma-Aldrich, Burlington, MA, United States) supplemented with 10% heat-inactivated bovine calf serum, 100 units/ml penicillin, and 100 μg/ml streptomycin (Sigma-Aldrich, Burlington, MA, United States) at 37°C in a humidified atmosphere of 5% CO2.

    Techniques: Incubation, Radioactivity, Protein Concentration, In Vivo, Injection

    Micro-PET imaging of NS3/4A activity in vivo . (A) Mice bearing established NS3/4A-HCC36 (right hind leg) and HCC36 (left hind leg) tumors were injected with 3,700 kBq of TAT-ΔNS3/4A- 124 I-FITC. Coronal and transverse images were acquired at 2, 4, and 6 h after injection of the probe. (B) Mice bearing established NS3/4A-HCC36 (right hind leg) and HCC36 (left hind leg) tumors were intraperitoneally injected with telaprevir (20 mg/kg/day for 3 days) before intravenous injection of TAT-ΔNS3/4A- 124 I-FITC (3,700 kBq). Coronal images of tumor sections were acquired at 2, 4, and 6 h after injection of the probe.

    Journal: Frontiers in Microbiology

    Article Title: Micro-PET imaging of hepatitis C virus NS3/4A protease activity using a protease-activatable retention probe

    doi: 10.3389/fmicb.2022.896588

    Figure Lengend Snippet: Micro-PET imaging of NS3/4A activity in vivo . (A) Mice bearing established NS3/4A-HCC36 (right hind leg) and HCC36 (left hind leg) tumors were injected with 3,700 kBq of TAT-ΔNS3/4A- 124 I-FITC. Coronal and transverse images were acquired at 2, 4, and 6 h after injection of the probe. (B) Mice bearing established NS3/4A-HCC36 (right hind leg) and HCC36 (left hind leg) tumors were intraperitoneally injected with telaprevir (20 mg/kg/day for 3 days) before intravenous injection of TAT-ΔNS3/4A- 124 I-FITC (3,700 kBq). Coronal images of tumor sections were acquired at 2, 4, and 6 h after injection of the probe.

    Article Snippet: Human hepatocellular carcinoma cells HCC36 (American Type Culture Collection, Manassas, VA, United States) were cultured in Dulbecco’s Minimal Essential Medium (Sigma-Aldrich, Burlington, MA, United States) supplemented with 10% heat-inactivated bovine calf serum, 100 units/ml penicillin, and 100 μg/ml streptomycin (Sigma-Aldrich, Burlington, MA, United States) at 37°C in a humidified atmosphere of 5% CO2.

    Techniques: Micro-PET, Imaging, Activity Assay, In Vivo, Injection

    Biodistribution of TAT-ΔNS3/4A- 124 I-FITC in xenograft mice. Mice bearing established NS3/4A-HCC36 and HCC36 tumors were injected with 3,700 kBq of TAT-ΔNS3/4A- 124 I-FITC. Selected organs and tumors were removed from the mice after 2 (white column), 4 (black column), and 6 (gray column) h. The radioactivity of individual organs was measured using a gamma-counter and normalized for sample weights. The biodistribution of TAT-ΔNS3/4A- 124 I-FITC in selected organs was expressed as percentage injected dose/g tissue. Data represent mean ± SEM.

    Journal: Frontiers in Microbiology

    Article Title: Micro-PET imaging of hepatitis C virus NS3/4A protease activity using a protease-activatable retention probe

    doi: 10.3389/fmicb.2022.896588

    Figure Lengend Snippet: Biodistribution of TAT-ΔNS3/4A- 124 I-FITC in xenograft mice. Mice bearing established NS3/4A-HCC36 and HCC36 tumors were injected with 3,700 kBq of TAT-ΔNS3/4A- 124 I-FITC. Selected organs and tumors were removed from the mice after 2 (white column), 4 (black column), and 6 (gray column) h. The radioactivity of individual organs was measured using a gamma-counter and normalized for sample weights. The biodistribution of TAT-ΔNS3/4A- 124 I-FITC in selected organs was expressed as percentage injected dose/g tissue. Data represent mean ± SEM.

    Article Snippet: Human hepatocellular carcinoma cells HCC36 (American Type Culture Collection, Manassas, VA, United States) were cultured in Dulbecco’s Minimal Essential Medium (Sigma-Aldrich, Burlington, MA, United States) supplemented with 10% heat-inactivated bovine calf serum, 100 units/ml penicillin, and 100 μg/ml streptomycin (Sigma-Aldrich, Burlington, MA, United States) at 37°C in a humidified atmosphere of 5% CO2.

    Techniques: Injection, Radioactivity

    Micro-PET Imaging of NS3/4A activity in orthotopic liver implantation model. The NS3/4A-HCC36 tumors or HCC36 tumors from the ectopic tumors were harvested and transplanted into the left liver lobe of SCID mice ( n = 3). After 2 weeks, the 3,700 kBq of TAT-ΔNS3/4A- 124 I-FITC was intravenously injected. (A) PET imaging was performed at 4 h after injection of the probe. (B) The accumulation of radioactivity in NS3/4A-HCC36 tumors and HCC36 tumors was measured using a gamma-counter at 2 (white column), 4 (black column), and 6 (gray column) h after probe injection. Data represent mean ± SEM. Student’s t -test analysis of data. Statistical analysis was compared NS3/4A-HCC36 with HCC36. P < 0.05 was considered statistically significant.

    Journal: Frontiers in Microbiology

    Article Title: Micro-PET imaging of hepatitis C virus NS3/4A protease activity using a protease-activatable retention probe

    doi: 10.3389/fmicb.2022.896588

    Figure Lengend Snippet: Micro-PET Imaging of NS3/4A activity in orthotopic liver implantation model. The NS3/4A-HCC36 tumors or HCC36 tumors from the ectopic tumors were harvested and transplanted into the left liver lobe of SCID mice ( n = 3). After 2 weeks, the 3,700 kBq of TAT-ΔNS3/4A- 124 I-FITC was intravenously injected. (A) PET imaging was performed at 4 h after injection of the probe. (B) The accumulation of radioactivity in NS3/4A-HCC36 tumors and HCC36 tumors was measured using a gamma-counter at 2 (white column), 4 (black column), and 6 (gray column) h after probe injection. Data represent mean ± SEM. Student’s t -test analysis of data. Statistical analysis was compared NS3/4A-HCC36 with HCC36. P < 0.05 was considered statistically significant.

    Article Snippet: Human hepatocellular carcinoma cells HCC36 (American Type Culture Collection, Manassas, VA, United States) were cultured in Dulbecco’s Minimal Essential Medium (Sigma-Aldrich, Burlington, MA, United States) supplemented with 10% heat-inactivated bovine calf serum, 100 units/ml penicillin, and 100 μg/ml streptomycin (Sigma-Aldrich, Burlington, MA, United States) at 37°C in a humidified atmosphere of 5% CO2.

    Techniques: Micro-PET, Imaging, Activity Assay, Injection, Radioactivity